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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two more splice variants had been claimed but thorough nucleotide sequence info just isn't nonetheless offered.

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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged via the antibody.

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This guidebook outlines more possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining from the blocked antibody vs . the antibody by itself, one can see which staining is certain.

Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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